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Hplc Column

January 23rd, 2010 Leave a comment Go to comments

Hplc Column
What are the chemical interactions occurring in a Reverse phase HPLC column?

I know that the reverse column is mostly nonpolar but how does that react with the analyte of interest and the speed of which eluent comes out first?

its called a hydrophobic interaction. non-polar regions of the analyte molecule adsorbs to the nonpolar media because there is a free-energy advantage to keeping water away. as you run a gradient and increase the concentration of organic, the adsorption becomes gradually less favorable. the least hydrophobic things elute first... the most hydrophobic things elute last

High(er) Performance Liquid Chromatography - HPLC


SPECTRA-PHYSICS SP8792 COLUMN HEATER HPLC


SPECTRA-PHYSICS SP8792 COLUMN HEATER HPLC


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ZORBAX SB-PHENYL  HPLC COLUMN ANALYTICAL GUARD  4.6- 12.5mm 5-mikron  #7173


ZORBAX SB-PHENYL HPLC COLUMN ANALYTICAL GUARD 4.6- 12.5mm 5-mikron #7173


$29.00


AGILENT ZORBAX HPLC ANALYTICAL GUARD COLUMN RX-C8 4- PACK 4.6- 12.5mm   #7172


AGILENT ZORBAX HPLC ANALYTICAL GUARD COLUMN RX-C8 4- PACK 4.6- 12.5mm #7172


$29.00


ZORBAX HPLC ANALYTICAL GUARD COLUMN 300SB-C18  4.6-12.5mm 5-mikron   #7182


ZORBAX HPLC ANALYTICAL GUARD COLUMN 300SB-C18 4.6-12.5mm 5-mikron #7182


$29.00


Waters Column Heater for Waters 600E HPLC Pump


Waters Column Heater for Waters 600E HPLC Pump


$295.00


Prozyme GlycoSep N Guard Column HPLC GKI-4728G NEW 4.6 x 10 mm


Prozyme GlycoSep N Guard Column HPLC GKI-4728G NEW 4.6 x 10 mm


$145.00


KROMASIL K40296 ANZO NOBEL 100-5-C18 5µm 100Å PORE SIZE 21.2 X 100mm HPLC COLUMN


KROMASIL K40296 ANZO NOBEL 100-5-C18 5µm 100Å PORE SIZE 21.2 X 100mm HPLC COLUMN


$1,299.99


KROMASIL K40271 ANZO NOBEL 100-5-C18 5µm 100Å PORE SIZE 30 X 150mm HPLC COLUMN


KROMASIL K40271 ANZO NOBEL 100-5-C18 5µm 100Å PORE SIZE 30 X 150mm HPLC COLUMN


$1,899.99


New HPLC  column SepaxHP-SCX cation exchange 2.1x50 mm 120363-2105 120A sepax


New HPLC column SepaxHP-SCX cation exchange 2.1x50 mm 120363-2105 120A sepax


$193.80


New HPLC  column PolyLC Polyhydroxyethyl   100x2.1 mm 5 um 100A P102HY0501


New HPLC column PolyLC Polyhydroxyethyl 100x2.1 mm 5 um 100A P102HY0501


$193.80


New HPLC  column PolyLC Polyhydroxyethyl A  100x4.6 mm 5 um 100A 104HY0501


New HPLC column PolyLC Polyhydroxyethyl A 100x4.6 mm 5 um 100A 104HY0501


$224.40


Agilent Zorbax 300SB-C8 5um 2.1x150mm PN: 883750-906 HPLC Column Sealed box


Agilent Zorbax 300SB-C8 5um 2.1x150mm PN: 883750-906 HPLC Column Sealed box


$218.00


Phenomenex Lichrosphere 5 CN HPLC Column 00F-3053-E0


Phenomenex Lichrosphere 5 CN HPLC Column 00F-3053-E0


$469.26


 Daicel Chiralcel OD-3, 4.6 x 150mm chiral HPLC column


Daicel Chiralcel OD-3, 4.6 x 150mm chiral HPLC column


$350.00


Agilent ZORBAX 300SB-CN 3.5um, 4.6 x 250mm HPLC Column Sealed box custom packing


Agilent ZORBAX 300SB-CN 3.5um, 4.6 x 250mm HPLC Column Sealed box custom packing


$225.00


 Daicel Chiralcel IA-3, 4.6 x 150mm chiral HPLC column


Daicel Chiralcel IA-3, 4.6 x 150mm chiral HPLC column


$350.00


 Daicel Chiralcel AD-3, 4.6 x 150mm chiral HPLC column


Daicel Chiralcel AD-3, 4.6 x 150mm chiral HPLC column


$350.00


H129364 Fiatron TC-50 Temperature Controller w/ HPLC Column Heater


H129364 Fiatron TC-50 Temperature Controller w/ HPLC Column Heater


$100.00


Waters HPLC Column YMC-Pack TMS TM12S03-1546WT A102-3 12mm 150-4.6 New #7149


Waters HPLC Column YMC-Pack TMS TM12S03-1546WT A102-3 12mm 150-4.6 New #7149


$169.00


TosoHaas TSK-Gel Super-ODS HPLC Column 4.6mm x 10cm #18197


TosoHaas TSK-Gel Super-ODS HPLC Column 4.6mm x 10cm #18197


$299.00


Alltech HPLC Column Pre-Saturation Silica Cartridges 4/PKG 28652


Alltech HPLC Column Pre-Saturation Silica Cartridges 4/PKG 28652


$489.40


Tested YMC ODS-A S3, 4.6 x 150mm, 120A HPLC column; p/n: AA12S031546WT


Tested YMC ODS-A S3, 4.6 x 150mm, 120A HPLC column; p/n: AA12S031546WT


$70.00


LKB BROMMA CHROMATOGRAPHY COLUMN HPLC MINT IN BOX RARE USED HARD TO FIND #1


LKB BROMMA CHROMATOGRAPHY COLUMN HPLC MINT IN BOX RARE USED HARD TO FIND #1


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Phenomenex Aqua C18, 30 x 4.6mm, 5u, 125A HPLC column; 03A-4299-E0


Phenomenex Aqua C18, 30 x 4.6mm, 5u, 125A HPLC column; 03A-4299-E0


$40.00


LKB BROMMA CHROMATOGRAPHY COLUMN HPLC MINT IN BOX RARE USED HARD TO FIND #2


LKB BROMMA CHROMATOGRAPHY COLUMN HPLC MINT IN BOX RARE USED HARD TO FIND #2


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Lot of 5 used HPLC columns


Lot of 5 used HPLC columns


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HPLC column Regis val-u-pak ODS 4.6x250 mm 5um 731900


HPLC column Regis val-u-pak ODS 4.6x250 mm 5um 731900


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HPLC column Alltech C18 4.6x250 mm


HPLC column Alltech C18 4.6x250 mm


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WATERS POLARITY 186001311 POLARIS dC18 5µm 3.0 X 250mm HPLC COLUMN


WATERS POLARITY 186001311 POLARIS dC18 5µm 3.0 X 250mm HPLC COLUMN


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THERMO Hypersil Gold C18 HPLC Column 2.1X50mm 1.9um 25002-052130


THERMO Hypersil Gold C18 HPLC Column 2.1X50mm 1.9um 25002-052130


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Phenomenex 00B-4336-Y0 Synergi™ 4 µm Polar-RP 80 Å, HPLC Column 50 x 3 mm, New i


Phenomenex 00B-4336-Y0 Synergi™ 4 µm Polar-RP 80 Å, HPLC Column 50 x 3 mm, New i


$110.00


Phenomenex 00B-4372-B0 Synergi Max-RP 100A 2.5 um  HPLC column 50x2 mm


Phenomenex 00B-4372-B0 Synergi Max-RP 100A 2.5 um HPLC column 50x2 mm


$37.49


Varian  2031 100x030  Polaris C8-Ether 3u HPLC Column


Varian 2031 100x030 Polaris C8-Ether 3u HPLC Column


$24.99


XBridge BEH Phenyl HPLC Column 130Å 3.5 µm, 4.6 mm X 50 mm, 186003332


XBridge BEH Phenyl HPLC Column 130Å 3.5 µm, 4.6 mm X 50 mm, 186003332


$38.99


Dynamax / Varian R00083101C Si, 4.6x250mm 60A HPLC column w/Guard; G911133


Dynamax / Varian R00083101C Si, 4.6x250mm 60A HPLC column w/Guard; G911133


$45.00


HP Agilent 1100 HPLC G1316A ColComp Thermostatted Column Compartment with valve


HP Agilent 1100 HPLC G1316A ColComp Thermostatted Column Compartment with valve


$950.00


HP Agilent 1100 HPLC G1316A ColComp Thermostatted Column Compartment


HP Agilent 1100 HPLC G1316A ColComp Thermostatted Column Compartment


$850.00


Waters uBondapak Phenyl, 3.9 x 300mm HPLC column; P/N WAT027198


Waters uBondapak Phenyl, 3.9 x 300mm HPLC column; P/N WAT027198


$45.00


YMC Pack SIL 06 HPLC Column  4.6 x 250mm SL6S05-2546WT


YMC Pack SIL 06 HPLC Column 4.6 x 250mm SL6S05-2546WT


$299.00


AMINEX HPLC COLUMN, HPX-87H, 300 x 7.8 mm,


AMINEX HPLC COLUMN, HPX-87H, 300 x 7.8 mm,


$950.00


Waters uBondapak C18, 3.9 x 300mm HPLC column; P/N 27324


Waters uBondapak C18, 3.9 x 300mm HPLC column; P/N 27324


$45.00


Waters Nova-Pak C18 3.9 x 150mm HPLC column; P/N 86344


Waters Nova-Pak C18 3.9 x 150mm HPLC column; P/N 86344


$45.00


Agilent Zorbax SB-CN 4.6 x 250mm, 5u HPLC column; P/N 880975-905


Agilent Zorbax SB-CN 4.6 x 250mm, 5u HPLC column; P/N 880975-905


$45.00


SynChropak Eprogen MB2SCD100-10 Micra HPLC Column SCD100 5u 100x2.1mm NEW


SynChropak Eprogen MB2SCD100-10 Micra HPLC Column SCD100 5u 100x2.1mm NEW


$645.20


Keystone Scientific 055-702-3 Betasil C8 HPLC Column 50x3mm 5u 100A


Keystone Scientific 055-702-3 Betasil C8 HPLC Column 50x3mm 5u 100A


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Alltech 8376 HPLC Column Spherisorb Silica 5u Female (B) 250x4.6mm B33


Alltech 8376 HPLC Column Spherisorb Silica 5u Female (B) 250x4.6mm B33


$1,142.29


Tosoh 05764 TSKgel G5000PW HPLC Column


Tosoh 05764 TSKgel G5000PW HPLC Column


$89.99


Varian A2000150x046  Polaris C18-A 5μ HPLC Column, 150mm x 4.6mm


Varian A2000150x046 Polaris C18-A 5μ HPLC Column, 150mm x 4.6mm


$24.99


Varian A2001150x046  Polaris C18-A 3μ HPLC Column, 150mm x 4.6mm


Varian A2001150x046 Polaris C18-A 3μ HPLC Column, 150mm x 4.6mm


$24.99


YMC-Pack Ph HPLC Column 4.6 x 250mm S-5 um A-403 # PH12S05-2546WT


YMC-Pack Ph HPLC Column 4.6 x 250mm S-5 um A-403 # PH12S05-2546WT


$179.00


YMC-Pack ODS-AQ HPLC Column 100x4.6mm ID S-5U  120A AQ12S05-1046WT NEW NOS $99


YMC-Pack ODS-AQ HPLC Column 100x4.6mm ID S-5U 120A AQ12S05-1046WT NEW NOS $99


$99.00


YMC HPLC column YMC-Pack Diol-300 300x8ml 300a 5um 30nm DL30S05-3008WT


YMC HPLC column YMC-Pack Diol-300 300x8ml 300a 5um 30nm DL30S05-3008WT


$262.50


 Waters IC-Pak Ion exclusion 7.8x150mm HPLC column WAT010295 7um


Waters IC-Pak Ion exclusion 7.8x150mm HPLC column WAT010295 7um


$262.50


VYDAC 218TP1010 HPLC Column  911125-18-10 PROTEIN PEPTIDE C18 NEW  NOS $299


VYDAC 218TP1010 HPLC Column 911125-18-10 PROTEIN PEPTIDE C18 NEW NOS $299


$299.00


HPLC column Agilent Varian Dynamax Stainless Steel 41.4mm ID End Fit Kit, R00008


HPLC column Agilent Varian Dynamax Stainless Steel 41.4mm ID End Fit Kit, R00008


$1,473.90


Waters Wat086344  Nova Pak HPLC Column 14 u C18 60 Â 3.9 x 150 mm


Waters Wat086344 Nova Pak HPLC Column 14 u C18 60 Â 3.9 x 150 mm


$189.50


ANALYTICAL BIOTRAP 500 C18 4.0 X 20mm HPLC COLUMN CHROMTECH


ANALYTICAL BIOTRAP 500 C18 4.0 X 20mm HPLC COLUMN CHROMTECH


$29.99


THERMO HYPERSIL 70105-052130 BETASIL C18 5µm 50 X 2.1mm HPLC COLUMN


THERMO HYPERSIL 70105-052130 BETASIL C18 5µm 50 X 2.1mm HPLC COLUMN


$39.99


Alltech Alltima HP C18 HL HPLC Column 5µm 4.6 x 250mm  #87698


Alltech Alltima HP C18 HL HPLC Column 5µm 4.6 x 250mm #87698


$54.95


YMC ODS-A 4.0 mm x 150mm 5 um 120A HPLC Column # AA12S05 1506WT


YMC ODS-A 4.0 mm x 150mm 5 um 120A HPLC Column # AA12S05 1506WT


$199.00


Waters Delta-Pak C18, 3.9 x 30cm, 300A HPLC column


Waters Delta-Pak C18, 3.9 x 30cm, 300A HPLC column


$175.00


E L INDUSTRIES 112221-AQSAQUASEP 5µm 100Å 50 X 2.0mm HPLC COLUMN


E L INDUSTRIES 112221-AQSAQUASEP 5µm 100Å 50 X 2.0mm HPLC COLUMN


$39.99


Phenomenex Polysep-GFC-P HPLC Column 300 x 7.8mm 00H-3143-K0


Phenomenex Polysep-GFC-P HPLC Column 300 x 7.8mm 00H-3143-K0


$50.00


GL Sciences Inertsil HPLC Column C8-3 3um -- 5020-04842 -- New


GL Sciences Inertsil HPLC Column C8-3 3um -- 5020-04842 -- New


$80.00


SUPELCO 15101AST 5µm ASTEC CHIROBIOTIC HPLC COLUMN 2cm X 1.0mm


SUPELCO 15101AST 5µm ASTEC CHIROBIOTIC HPLC COLUMN 2cm X 1.0mm


$129.99


Agilent Technologies 827700-905 HPLC Column Rapid Resolution HT Zorbax SB (1945)


Agilent Technologies 827700-905 HPLC Column Rapid Resolution HT Zorbax SB (1945)


$165.75


PRONTOSIL 0502 F18APS030 120-3-C18-ACE-EPS 3µm 50 X 2.0mm HPLC COLUMN


PRONTOSIL 0502 F18APS030 120-3-C18-ACE-EPS 3µm 50 X 2.0mm HPLC COLUMN


$39.99


Tested Waters Symmetry C18 MV Kit, 4.6 x 150mm, 3.5u HPLC column; WAT094240


Tested Waters Symmetry C18 MV Kit, 4.6 x 150mm, 3.5u HPLC column; WAT094240


$75.00


YMC Pack Pro C18 4.0 x 150mm 5 um HPLC Column # AS12S051504WT


YMC Pack Pro C18 4.0 x 150mm 5 um HPLC Column # AS12S051504WT


$229.00


HPLC Column Phenomenex Luna 3 µm Phenyl-Hexyl 100 Å, 50 x 3 mm 00B-4256-Y0


HPLC Column Phenomenex Luna 3 µm Phenyl-Hexyl 100 Å, 50 x 3 mm 00B-4256-Y0


$238.00


HPLC Column Phenomenex Sphereclone 3u ODS(1) HPLC Column 00F-4134-R0 150x3.20mm


HPLC Column Phenomenex Sphereclone 3u ODS(1) HPLC Column 00F-4134-R0 150x3.20mm


$198.00


SecurityGuard cartridges for Gemini C6-Phenyl HPLC columns with 2.0 to 3.0mm ID


SecurityGuard cartridges for Gemini C6-Phenyl HPLC columns with 2.0 to 3.0mm ID


$70.00


HPLC Column Phenomenex Synergi™ 2.5 µm Max-RP 100 Å, 00B-4372-B0 50x2 mm


HPLC Column Phenomenex Synergi™ 2.5 µm Max-RP 100 Å, 00B-4372-B0 50x2 mm


$228.00


New HPLC column Agilent Zorbax 300SB-C18 5 um 0.5x35 mm 5064-8295


New HPLC column Agilent Zorbax 300SB-C18 5 um 0.5x35 mm 5064-8295


$85.00


ACE 3 C8, 150 x 4.6mm HPLC column; ACE-112-1546


ACE 3 C8, 150 x 4.6mm HPLC column; ACE-112-1546


$135.00


ACE 3 C8, 150 x 4.6mm HPLC column; ACE-112-1546


ACE 3 C8, 150 x 4.6mm HPLC column; ACE-112-1546


$95.00


Vydac 218TP54 HPLC Column


Vydac 218TP54 HPLC Column


$24.99


ACE 3 C8, 150 x 2.1mm HPLC column; ACE-112-1502


ACE 3 C8, 150 x 2.1mm HPLC column; ACE-112-1502


$95.00


Peeke Scientific Ultro 500 C4 150 x 2.1 mm ID HPLC Column   P/N  U5-5C4-DJ


Peeke Scientific Ultro 500 C4 150 x 2.1 mm ID HPLC Column P/N U5-5C4-DJ


$0.99


New HPLC column Thermo Electron Hypersil  BDS C18  2.1x20 mm  3 um  023-46-2


New HPLC column Thermo Electron Hypersil BDS C18 2.1x20 mm 3 um 023-46-2


$49.00


New HPLC column Thermo Electron BioBasic 1x20 mm  5 um  025-731-1 bio basic


New HPLC column Thermo Electron BioBasic 1x20 mm 5 um 025-731-1 bio basic


$49.00


New HPLC column Thermo Electron BioBasic AX 2x20 mm  5 um  025-731-2 bio basic


New HPLC column Thermo Electron BioBasic AX 2x20 mm 5 um 025-731-2 bio basic


$49.00


New HPLC column Keystone WAX 1x20 mm  5 um  cat number 025-ZZZ-1,  300A


New HPLC column Keystone WAX 1x20 mm 5 um cat number 025-ZZZ-1, 300A


$43.00


New HPLC column Thermo Electron BioBasic AX 0.3x50 mm  5 um  025-731-C3 basic


New HPLC column Thermo Electron BioBasic AX 0.3x50 mm 5 um 025-731-C3 basic


$49.00


ACE 3 C18, 50 x 1.0mm HPLC column; ACE-111-0501


ACE 3 C18, 50 x 1.0mm HPLC column; ACE-111-0501


$55.00


New HPLC column Thermo Electronl  Hypersil 2x50 mm  5 um  BetaMax base 055-951-2


New HPLC column Thermo Electronl Hypersil 2x50 mm 5 um BetaMax base 055-951-2


$49.00


New HPLC column Thermo Electron BioBasic AX 0.3x100 mm  5 um  105-731-C3 basic


New HPLC column Thermo Electron BioBasic AX 0.3x100 mm 5 um 105-731-C3 basic


$49.00


ACE 3 C18-AR, 50 x 2.1mm HPLC column; ACE-119-0502


ACE 3 C18-AR, 50 x 2.1mm HPLC column; ACE-119-0502


$75.00


HPLC COLUMN, Waters Atlantis T3, IS, 2.1 mm x 20 mm, NIB SEALED, 186003715


HPLC COLUMN, Waters Atlantis T3, IS, 2.1 mm x 20 mm, NIB SEALED, 186003715


$199.95


New Phenomenex Ultracarb 3 ODS(20), 80 x 6.2mm HPLC Column


New Phenomenex Ultracarb 3 ODS(20), 80 x 6.2mm HPLC Column


$135.00


Phenomenex Jupiter C4 column HPLC 4.6x250 mm  5 um  00G-4167-E0 300A sxzd


Phenomenex Jupiter C4 column HPLC 4.6x250 mm 5 um 00G-4167-E0 300A sxzd


$55.00


Phenomenex Jupiter C18 column HPLC 4.6x250 mm  5 um  00G-4053-E0 300A sxzd


Phenomenex Jupiter C18 column HPLC 4.6x250 mm 5 um 00G-4053-E0 300A sxzd


$59.00


Phenomenex Jupiter Proteo  column HPLC 4.6x250 mm  4 um  00G-4396-E0 90A vbtg


Phenomenex Jupiter Proteo column HPLC 4.6x250 mm 4 um 00G-4396-E0 90A vbtg


$70.00


HPLC column Regis Reversible octyl Spherisorb S5C8


HPLC column Regis Reversible octyl Spherisorb S5C8


$95.00


 HPLC column  Varian Metachem Polaris 5 um  C18-A  50


HPLC column Varian Metachem Polaris 5 um C18-A 50


$57.00


 HPLC column  Varian Metachem Polaris 3 um  C18-A  50


HPLC column Varian Metachem Polaris 3 um C18-A 50


$57.00


Keystone Hypersil BDS C18 HPLC Column 100x 4.6mm 5μm #105-46


Keystone Hypersil BDS C18 HPLC Column 100x 4.6mm 5μm #105-46


$59.95


HPLC column Waters Symmetry C-18 3.5um 2.1mmx50mm


HPLC column Waters Symmetry C-18 3.5um 2.1mmx50mm


$119.00


Phenomenex   column HPLC Aqua C18 00G-4299-E0 4.6x250 mm  5 um  125A


Phenomenex column HPLC Aqua C18 00G-4299-E0 4.6x250 mm 5 um 125A


$65.00


HPLC column Restek Ultra Cyano 3 um 50 x 3.2 cartridge


HPLC column Restek Ultra Cyano 3 um 50 x 3.2 cartridge


$145.00

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