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Hplc Column

January 23rd, 2010 Leave a comment Go to comments

Hplc Column
What are the chemical interactions occurring in a Reverse phase HPLC column?

I know that the reverse column is mostly nonpolar but how does that react with the analyte of interest and the speed of which eluent comes out first?

its called a hydrophobic interaction. non-polar regions of the analyte molecule adsorbs to the nonpolar media because there is a free-energy advantage to keeping water away. as you run a gradient and increase the concentration of organic, the adsorption becomes gradually less favorable. the least hydrophobic things elute first... the most hydrophobic things elute last

High(er) Performance Liquid Chromatography - HPLC


Tested Zorbax 300SB-C8, 4.6 x 250mm HPLC column; p/n 880995-906


Tested Zorbax 300SB-C8, 4.6 x 250mm HPLC column; p/n 880995-906


$95.00


Waters Millipore Protein Pak 300 SW Column 7.5 x 300 mm 080013 (HPLC Flow Colum)


Waters Millipore Protein Pak 300 SW Column 7.5 x 300 mm 080013 (HPLC Flow Colum)


$137.50


Tested Waters SymmetryShield RP8, 4.6 x 150mm HPLC column; WAT200662


Tested Waters SymmetryShield RP8, 4.6 x 150mm HPLC column; WAT200662


$85.00


Waters Millipore Protein Pak 300 SW Column 7.5 x 300 mm 080013 (HPLC Colum) #11


Waters Millipore Protein Pak 300 SW Column 7.5 x 300 mm 080013 (HPLC Colum) #11


$150.00


Hitachi Type L-5025 HPLC Column Thermostat


Hitachi Type L-5025 HPLC Column Thermostat


$104.98


Waters Millipore Protein Pak 60 Column 7.8 x 300 mm 085250 (HPLC Colum) #11


Waters Millipore Protein Pak 60 Column 7.8 x 300 mm 085250 (HPLC Colum) #11


$150.00


Waters TCM CHM Temperature Control Module & Column Heater HPLC #11


Waters TCM CHM Temperature Control Module & Column Heater HPLC #11


$399.50


VARIAN - POLARIS 5 C8-A 5u 50x2.0mm HPLC COLUMN / P/N: A2010-050X020


VARIAN - POLARIS 5 C8-A 5u 50x2.0mm HPLC COLUMN / P/N: A2010-050X020


$300.00


Phenomenex BioSep-SEC-S 3000 300x7.80mm HPLC Column P/N : 00H-2146-K0


Phenomenex BioSep-SEC-S 3000 300x7.80mm HPLC Column P/N : 00H-2146-K0


$901.00


Tested Phenomenex Ultracarb 5 ODS(30), 150 x 4.6mm, 5u HPLC column; 00F-0351-E0


Tested Phenomenex Ultracarb 5 ODS(30), 150 x 4.6mm, 5u HPLC column; 00F-0351-E0


$70.00


Anspec Synchron H2057 HPLC Column 250x4.1mm ID Synchropak CM300


Anspec Synchron H2057 HPLC Column 250x4.1mm ID Synchropak CM300


$123.88


Waters Column Heater Model Code SMH (HPLC)


Waters Column Heater Model Code SMH (HPLC)


$299.99


Alltech HPLC Column Hypersil BDS-C8 5U


Alltech HPLC Column Hypersil BDS-C8 5U


$0.99


Agilent Zorbax 300SB-C18 3.5um HPLC Column


Agilent Zorbax 300SB-C18 3.5um HPLC Column


$0.99


Varian Chromsep HPLC Column SS 3


Varian Chromsep HPLC Column SS 3


$0.99


Agilent Zorbax 300SB-C18 3.5um, 150x0, .1mm HPLC Column


Agilent Zorbax 300SB-C18 3.5um, 150x0, .1mm HPLC Column


$0.99


Agilent Zorbax 300-C18 3.5um, 150x0, .075mm HPLC Column


Agilent Zorbax 300-C18 3.5um, 150x0, .075mm HPLC Column


$0.99


Shimadzu sample cooler chiller HPLC column cooling chilling chromatography


Shimadzu sample cooler chiller HPLC column cooling chilling chromatography


$199.00


TosoHaas 13076 TSKgel SP-NPR HPLC Column 3.5 cm × 4.6 mm, 2.5 μm particle size


TosoHaas 13076 TSKgel SP-NPR HPLC Column 3.5 cm × 4.6 mm, 2.5 μm particle size


$160.00


TosoHaas 13076 TSKgel SP-NPR HPLC Column 3.5 cm × 4.6 mm, 2.5 μm particle size


TosoHaas 13076 TSKgel SP-NPR HPLC Column 3.5 cm × 4.6 mm, 2.5 μm particle size


$80.00


HPLC COLUMN, Phenomenex Luna C5, 4.6 x 150 mm, 5 um, 00F-4043-E0


HPLC COLUMN, Phenomenex Luna C5, 4.6 x 150 mm, 5 um, 00F-4043-E0


$69.95


HPLC COLUMN, Waters Symmetry C18, 4.6 x 150 mm, 3.5 um, WAT200632 Chromatography


HPLC COLUMN, Waters Symmetry C18, 4.6 x 150 mm, 3.5 um, WAT200632 Chromatography


$49.95


HPLC COLUMN, Waters Atlantis dC18, 3.9 x 150 mm, NIB SEALED, 186001319, C18


HPLC COLUMN, Waters Atlantis dC18, 3.9 x 150 mm, NIB SEALED, 186001319, C18


$314.95


TosoHaas 07161 TSKgel SP-5PW 7.5mm ID x 7.5cm HPLC Chromatography column


TosoHaas 07161 TSKgel SP-5PW 7.5mm ID x 7.5cm HPLC Chromatography column


$160.00


HPLC COLUMN, Phenomenex Gemini C18, 4.6 x 50 mm, 3 um, 00B-4439-E0


HPLC COLUMN, Phenomenex Gemini C18, 4.6 x 50 mm, 3 um, 00B-4439-E0


$44.95


HPLC Part Hibar EM Science Pre-Packed Column RT 250-4 LiChrosorb CN (5um)


HPLC Part Hibar EM Science Pre-Packed Column RT 250-4 LiChrosorb CN (5um)


$50.00


HPLC  MetaChem Polygosil 5-C18 Column Part Waters


HPLC MetaChem Polygosil 5-C18 Column Part Waters


$50.00


HPLC COLUMN, Thermo BioBasic SCX, 0.32 mm x 100 mm, NIB SEALED, 73205-100367


HPLC COLUMN, Thermo BioBasic SCX, 0.32 mm x 100 mm, NIB SEALED, 73205-100367


$289.95


Grace Alltima HPLC Column 250x4.6 mm


Grace Alltima HPLC Column 250x4.6 mm


$44.99


New Sealed Agilent HPLC column Zorbax 300SB-C18 PN# 865750-902


New Sealed Agilent HPLC column Zorbax 300SB-C18 PN# 865750-902


$189.00


Waters 600E HPLC SHU fluid handling unit column heater + assembly


Waters 600E HPLC SHU fluid handling unit column heater + assembly


$405.09


New SEALED Agilent ZORBAX Eclipse XDB-C8 HPLC LC Column 4.6x150mm 5um 993967-906


New SEALED Agilent ZORBAX Eclipse XDB-C8 HPLC LC Column 4.6x150mm 5um 993967-906


$449.99


HP HPLC column Zorbax Eclipse XDB-C8 5 um  4.6x150 mm P/N: 993967-906


HP HPLC column Zorbax Eclipse XDB-C8 5 um 4.6x150 mm P/N: 993967-906


$200.00


LOT OF HPLC COLUMNS


LOT OF HPLC COLUMNS


$50.00


WATERS u-BONDAPAK C18 HPLC COLUMN 3.9x155mm 125A WAT086684


WATERS u-BONDAPAK C18 HPLC COLUMN 3.9x155mm 125A WAT086684


$27.00


PAIR- WHATMAN PARTISIL HPLC 10 SAX AND PARTISIL PXS 10/25 PAC COLUMNS


PAIR- WHATMAN PARTISIL HPLC 10 SAX AND PARTISIL PXS 10/25 PAC COLUMNS


$36.00


Agilent ZORBAX SB-CN HPLC LC Column 4.6mm x 250mm 5um P/N 880975-905


Agilent ZORBAX SB-CN HPLC LC Column 4.6mm x 250mm 5um P/N 880975-905


$149.99


Waters SunFire C18 HPLC LC Column 4.6mm x 150mm 3.5um P/N 186002554


Waters SunFire C18 HPLC LC Column 4.6mm x 150mm 3.5um P/N 186002554


$149.99


Agilent ZORBAX SB-Phenyl HPLC LC Column 4.6mm x 250mm 5um P/N 880975-912


Agilent ZORBAX SB-Phenyl HPLC LC Column 4.6mm x 250mm 5um P/N 880975-912


$149.99


Waters XBridge Phenyl HPLC LC Column 4.6mm x 250mm 5um P/N 186003353


Waters XBridge Phenyl HPLC LC Column 4.6mm x 250mm 5um P/N 186003353


$199.99


Waters SunFire C18 HPLC LC Column 4.6mm x 150mm 5um P/N 186002559


Waters SunFire C18 HPLC LC Column 4.6mm x 150mm 5um P/N 186002559


$149.99


Waters XBridge C18 HPLC LC Column 4.6mm x 250mm 5um P/N 186003117


Waters XBridge C18 HPLC LC Column 4.6mm x 250mm 5um P/N 186003117


$199.99


Alltech Alltima HP CN 5u 150mm x 4.6mm HPLC Columns P/N: 87769


Alltech Alltima HP CN 5u 150mm x 4.6mm HPLC Columns P/N: 87769


$400.00


2D HPLC COLUMN SWITCHING SYSTEM; Multidimensional Comprehensive, CS3020 In-Line


2D HPLC COLUMN SWITCHING SYSTEM; Multidimensional Comprehensive, CS3020 In-Line


$5,250.00


Waters Symmetry C8 5µm 4.6 x 250mm HPLC Column WAT054245


Waters Symmetry C8 5µm 4.6 x 250mm HPLC Column WAT054245


$99.95


WATERS Atlantis T3 HPLC Column, 100Å, 3 µm, 4.6 mm X 50 mm pn 186003726


WATERS Atlantis T3 HPLC Column, 100Å, 3 µm, 4.6 mm X 50 mm pn 186003726


$235.00


Vydac HPLC Column 301VHP5410 900Å 5µm polymer DEAE-type ion exchange 4.6mmx100mm


Vydac HPLC Column 301VHP5410 900Å 5µm polymer DEAE-type ion exchange 4.6mmx100mm


$165.00


Tosoh Super-ODS HPLC column 10 cm X 2.0 mm 2um #19542


Tosoh Super-ODS HPLC column 10 cm X 2.0 mm 2um #19542


$80.00


7pc Perkin Elmer Brownlee spheri-5 RP-18 5um c-18 22cm HPLC column cartridges


7pc Perkin Elmer Brownlee spheri-5 RP-18 5um c-18 22cm HPLC column cartridges


$65.00


YMC HYDROSPHERE C18 HS12S03-1546WT HS-302-3 3µm 12nm 4.6 X 150mm HPLC COLUMN


YMC HYDROSPHERE C18 HS12S03-1546WT HS-302-3 3µm 12nm 4.6 X 150mm HPLC COLUMN


$79.99


Tosoh Super-ODS HPLC column 5 cm X 2.0 mm #19541 used


Tosoh Super-ODS HPLC column 5 cm X 2.0 mm #19541 used


$80.00


NEW HPLC COLUMN, TSKGEL, Phenyl-5PW, 7.5mm x 7.5 cm, 10um Tosoh 07573


NEW HPLC COLUMN, TSKGEL, Phenyl-5PW, 7.5mm x 7.5 cm, 10um Tosoh 07573


$190.00


New Bio-Rad Bio-Sil SEC 250 HPLC Column 300 x 7.8mm Cat # 125-0062


New Bio-Rad Bio-Sil SEC 250 HPLC Column 300 x 7.8mm Cat # 125-0062


$395.00


Shimadzu CTO-20AC Column Oven - Prominence HPLC System LC 20A heater Col 10A vp


Shimadzu CTO-20AC Column Oven - Prominence HPLC System LC 20A heater Col 10A vp


$2,250.00


Phenomenex 00B-4439-B0 Gemini 3 um C18 50x2 mm HPLC Column


Phenomenex 00B-4439-B0 Gemini 3 um C18 50x2 mm HPLC Column


$50.00


Column Engineering inc. HPLC column Monitor 3u C18 50x2.0 mm  M3BE-001


Column Engineering inc. HPLC column Monitor 3u C18 50x2.0 mm M3BE-001


$49.99


Grace Alltech Lichroshere RP-8 3u, 250mm Length, 4.6mm ID, HPLC Column


Grace Alltech Lichroshere RP-8 3u, 250mm Length, 4.6mm ID, HPLC Column


$119.99


Tested Waters Symmetry C18, 3.9 x 150mm HPLC column; p/n WAT046980


Tested Waters Symmetry C18, 3.9 x 150mm HPLC column; p/n WAT046980


$85.00


Tested Phenomenex Prodigy ODS-2, 150 x 4.6mm, 5u HPLC column


Tested Phenomenex Prodigy ODS-2, 150 x 4.6mm, 5u HPLC column


$65.00


Tested Phenomenex IB-SIL Phenyl, 150 x 4.6mm, 3u HPLC column


Tested Phenomenex IB-SIL Phenyl, 150 x 4.6mm, 3u HPLC column


$65.00


Tested Supelco Supelcosil LC-18, 150 x 4.6mm, 3u HPLC column; Col 15599-004


Tested Supelco Supelcosil LC-18, 150 x 4.6mm, 3u HPLC column; Col 15599-004


$85.00


Waters WAT038040 HPLC Column Heater Module - WORKING UNIT


Waters WAT038040 HPLC Column Heater Module - WORKING UNIT


$299.99


Grace Alltech HPLC Column, Macrosphere GPC 4000 7U, 250mm x 4.6mm


Grace Alltech HPLC Column, Macrosphere GPC 4000 7U, 250mm x 4.6mm


$129.99


WATERS WAT094263 HPLC RP8 COLUMN *NEW NO BOX*


WATERS WAT094263 HPLC RP8 COLUMN *NEW NO BOX*


$195.00


Lot of HPLC Columns, Various Plugs, Guards See Description


Lot of HPLC Columns, Various Plugs, Guards See Description


$349.99


Thermo Scientific 502053-775 Aquasil C18 3um 50x2mm Hypersil HPLC Column


Thermo Scientific 502053-775 Aquasil C18 3um 50x2mm Hypersil HPLC Column


$250.00


Lot 2 Thermo Scientific 25005-103030 Hypersil Gold Dim (Mm) 100x3 HPLC Column


Lot 2 Thermo Scientific 25005-103030 Hypersil Gold Dim (Mm) 100x3 HPLC Column


$300.00


Zorbax 4.6 x 150mm 883952.70 HPLC Column


Zorbax 4.6 x 150mm 883952.70 HPLC Column


$49.99


08021 TSKgel® G3000PWXL HPLC Column


08021 TSKgel® G3000PWXL HPLC Column


$1,500.00


08540 TSKgel G2000SWXL HPLC Column


08540 TSKgel G2000SWXL HPLC Column


$1,500.00


18257 TSKgel SuperQ-5PW HPLC Column


18257 TSKgel SuperQ-5PW HPLC Column


$1,200.00


Waters 186001179 HPLC Column 2.1x50mm XTerra Phenyl 3.5um


Waters 186001179 HPLC Column 2.1x50mm XTerra Phenyl 3.5um


$52.49


YMC-Pack A-402 S-5 5um 4.6 x 150mm HPLC Chromatography Column PH12S05-1546WT


YMC-Pack A-402 S-5 5um 4.6 x 150mm HPLC Chromatography Column PH12S05-1546WT


$58.42


Tested Phenomenex Partisil ODS(3), 250 x 4.6mm, 5u HPLC column; 00G-0120-E0


Tested Phenomenex Partisil ODS(3), 250 x 4.6mm, 5u HPLC column; 00G-0120-E0


$80.00


Shodex 7810020 Asahipak ODP-50 4D HPLC Chromatography Column


Shodex 7810020 Asahipak ODP-50 4D HPLC Chromatography Column


$56.99


WATERS HPLC 25mm CARTRIDGE ADAPTER w COUPLING WAT015859 WAT015860 PrepLC COLUMN


WATERS HPLC 25mm CARTRIDGE ADAPTER w COUPLING WAT015859 WAT015860 PrepLC COLUMN


$30.00


YMC ODS-A S5µm 120A 4.6 X 50mm  AA12S050546WT HPLC Column


YMC ODS-A S5µm 120A 4.6 X 50mm AA12S050546WT HPLC Column


$68.88


Tested Inertsil C8-3, 5u, 3.0 x 250 mm HPLC column; 520-04926


Tested Inertsil C8-3, 5u, 3.0 x 250 mm HPLC column; 520-04926


$75.00


R119526 Phenomenex Luna 3u C18(2) p/n 00B-4251-AO HPLC Column


R119526 Phenomenex Luna 3u C18(2) p/n 00B-4251-AO HPLC Column


$100.00


R119525 Phenomenex Synergi 4u Max-RP 80A P/N 00B-4337-BO HPLC Column


R119525 Phenomenex Synergi 4u Max-RP 80A P/N 00B-4337-BO HPLC Column


$100.00


R119516 Agilent HPLC Column Zorbax SB-C8 Rapid Resolution HT 2.1x30mm 1.8-Micron


R119516 Agilent HPLC Column Zorbax SB-C8 Rapid Resolution HT 2.1x30mm 1.8-Micron


$100.00


R119512 Agilent HPLC Column Analytical 4.6x150mm 5-Micron p/n 993967-906


R119512 Agilent HPLC Column Analytical 4.6x150mm 5-Micron p/n 993967-906


$100.00


R119510 Agilent HPLC Column Zorbax SB-C8 LC-MS Column 2.1x50mm 5-Micron


R119510 Agilent HPLC Column Zorbax SB-C8 LC-MS Column 2.1x50mm 5-Micron


$100.00


R119508 Agilent HPLC Column Zorbax Eclipse  XDB-C8 Analytical 406x150mm 5-Micron


R119508 Agilent HPLC Column Zorbax Eclipse XDB-C8 Analytical 406x150mm 5-Micron


$100.00


R119507 Agilent HPLC Column Zorbax SB-C18 Analytical 406x50mm 5-Micron p/n 84697


R119507 Agilent HPLC Column Zorbax SB-C18 Analytical 406x50mm 5-Micron p/n 84697


$100.00


Tested Varian Inertsil 5 C8, 250 x 3.0 mm HPLC column; A0297250X030


Tested Varian Inertsil 5 C8, 250 x 3.0 mm HPLC column; A0297250X030


$75.00


Waters Nova-Pak C18, 3.9 x 150mm HPLC column; WAT036975


Waters Nova-Pak C18, 3.9 x 150mm HPLC column; WAT036975


$45.00


Waters HPLC SymmetryShield RP18 3.5 um 4.6 x 100 mm column


Waters HPLC SymmetryShield RP18 3.5 um 4.6 x 100 mm column


$350.00


Phenomenex Prodigy 5 µm ODS-3 100 Å HPLC Column 50 x 2 mm #00B-4097-B0


Phenomenex Prodigy 5 µm ODS-3 100 Å HPLC Column 50 x 2 mm #00B-4097-B0


$49.95


Waters Column Heater for Alliance HPLC 2695/2690/2795/2790 Nice and Clean


Waters Column Heater for Alliance HPLC 2695/2690/2795/2790 Nice and Clean


$1,099.99


Toso Haas TSKgel G3000SW HPLC Column 60cm × 7.5mm 10μm #05103


Toso Haas TSKgel G3000SW HPLC Column 60cm × 7.5mm 10μm #05103


$399.00


Vydac 214MS5110 C4 Mass Spec HPLC Column


Vydac 214MS5110 C4 Mass Spec HPLC Column


$38.88


R135257 Amicon 43


R135257 Amicon 43" Chromatography FPLC HPLC Column


$100.00


HPLC COLUMN, Phenomenex HyperClone MOS (C8), 4.6 x 125 mm, NIB, 00E-4359-E0


HPLC COLUMN, Phenomenex HyperClone MOS (C8), 4.6 x 125 mm, NIB, 00E-4359-E0


$159.95


Thermo Scientific 70203-052130 BetaSil C8 Analytical HPLC Column


Thermo Scientific 70203-052130 BetaSil C8 Analytical HPLC Column


$599.00


Thermo Fisher Scientific 73105-053030 Bio Basic AX Analytical HPLC Column


Thermo Fisher Scientific 73105-053030 Bio Basic AX Analytical HPLC Column


$499.00


HPLC COLUMN, Thermo BioBasic SEC-120, 7.8 mm x 30 mm, NIB, 73405-037821


HPLC COLUMN, Thermo BioBasic SEC-120, 7.8 mm x 30 mm, NIB, 73405-037821


$269.95


Water Atlantis HPLC Columns


Water Atlantis HPLC Columns


$99.00


HPLC COLUMN, Vydac C18, 2.1 x 250 mm, 218TP52, SEALED, Polymeric Reversed Phase


HPLC COLUMN, Vydac C18, 2.1 x 250 mm, 218TP52, SEALED, Polymeric Reversed Phase


$349.95


HPLC Columns


HPLC Columns


$120.00

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